Journal: Applications in Plant Sciences

Article Title: Barcoded NS31/AML2 primers for sequencing of arbuscular mycorrhizal communities in environmental samples ¹

doi: 10.3732/apps.1700017

Figure Lengend Snippet: Individual components and complete sequences of Illumina MiSeq-compatible custom sequencing primers used to sequence amplicon libraries in this study and their estimated melting temperatures.

Article Snippet: Libraries of small subunit ribosomal RNA (hereafter referred to as 18S) fragment amplicons were prepared for each sample with a single-step PCR reaction using primers NS31 ( ) and AML2 ( ) that we modified for use with Illumina sequencing platforms following the protocol of .

Techniques: Sequencing, Amplification, Construct

Journal: Applications in Plant Sciences

Article Title: Barcoded NS31/AML2 primers for sequencing of arbuscular mycorrhizal communities in environmental samples ¹

doi: 10.3732/apps.1700017

Figure Lengend Snippet: Twelve-base Golay error-correcting barcode sequences that passed in silico testing for secondary structure formation. Individual barcode sequences from this list are substituted for XXXXXXXXXXXX in the “Golay barcode” region ( Table 2 ) to generate indexed reverse primer constructs for PCR.

Article Snippet: Libraries of small subunit ribosomal RNA (hereafter referred to as 18S) fragment amplicons were prepared for each sample with a single-step PCR reaction using primers NS31 ( ) and AML2 ( ) that we modified for use with Illumina sequencing platforms following the protocol of .

Techniques: In Silico, Construct